Symptoms of poisoning may even occur after several hours. Practical products supplied by spectrum are indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or. How do you run protein samples in 6m guhcl in sdspage. Guanidine hydrochloride, practical, also known as guanidinium chloride, is the hydrochloride salt of guanidine used in physiochemical studies of protein folding. Guanidine hydrochloride is indicated for the reduction of the symptoms of muscle weakness and easy fatigability associated with eatonlambert syndrome. I have eluted this glycoprotein from the protein gbound antibody using 6 m guanidine hydrochloride. Carolina offers the highest quality kits for a handson approach within ap chemistry classrooms. Identification product name guanidine hydrochloride cat no. Extreme high moderate low section 1 chemical product and company identification product name guanidine thiocyanate statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910. Guanadine hcl is one of the strongest denaturing agents for protein folding studies, and is used to denature proteins due to its chaotropic qualities. Dec 02, 2019 fatal bonemarrow suppression, apparently dose related, can occur with guanidine. Therefore, the benefits of therapy must be weighed against the potential hazards. Any concentration below 8m guanidine can be quickly and ea.
Product name guanidine hydrochloride, for molecular biology 1. Each tablet contains 125 mg of guanidine hydrochloride with no color additive in the base. Being a so called chaotropic agent guanidine hcl is used as a powerful protein denaturant in the purification of proteins and nucleic acids dna and rna. Guanidinium chloride is a weak acid with a pk a of. Guanidine hydrochloride enhances the release of acetylcholine following a nerve impulse and potentiates acetylcholine actions on muscarinic and nicotinic receptors. As with most organic solids, fire is possible at elevated temperatures or by contact with an ignition source. Personal protective equipment eyeface protection safety glasses with sideshields conforming to en166 use equipment for eye protection tested and approved under appropriate government standards such as niosh us or en 166eu. Safety data sheet guanidine hydrochloride date prepared. Chemically, guanidine aminomethanamidine hydrochloride is a crystalline powder freely soluble in water and alcohol. The reason letting it such a weak acid is because of the complete delocalisation of the positive charge through 3 nitrogen atoms plus a little bit positive charge on carbon. Guanidinium thiocyanate an overview sciencedirect topics.
It apparently acts by enhancing the release of acetylcholine following a nerve impulse. Urea vs guanidine hydrochloride gdnhcl in equilibrium denaturation experiments, the concentration of urea at midpoint of denaturation is always higher than that of gdnhcl. Proteins become randomly coiled at high concentrations of guanidinium chloride. Thermo scientific pierce guanidine hcl can be easily dissolved and phadjusted to any concentration. Because guanidine is excreted in milk, patients on this drug should discontinue breastfeeding.
Safety data sheet according to federal register vol. Find patient medical information for guanidine oral on webmd including its uses, side effects and safety, interactions, pictures, warnings and user ratings. Othersguanidine hcl is the most popular protein denaturant. Guanidine hydrochloride is used for purification of proteins and nucleic acids. Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Articles of guanidine hydrochloride are included as well. Choose from over 850 chemical products in chemical grades, sizes and concentrations to meet your needs. It acts as an intermediate in the preparation of sulfadiazine, which is an important raw material for sulfamethyldiazine, sulfamethazine and folic acid. Spectrum offers highly pure reagents suitable for biochemical research and analysis. Guanidine hydrochloride cas no 50011 material safety data sheet sds msds section 1.
It also appears to slow the rates of depolarization and repolarization of muscle cell membranes. Guanidine hydrochloride is the hydrochloride salt form of guanidine, a strong basic compound with parasympathomimetic activity. However, some stronger bases can deprotonate it, such as sodium hydroxide. Safety data sheet creation date 16nov2010 revision date 18jan2018 revision number 3 1. Guanidine hydrochloride, biotechgrade is considered a chaotrope and a strong denaturants utilized in physiochemical studies of protein folding. Aminoformamidine hydrochloride, aminomethanamidine hydrochloride, guanidinium chloride cas number 50011. Guanidine hcl is used in the isolation of rna to dissociate the nucleoprotein into its nucleic acid and protein. The most effective method for inactivating endogenous nucleases and deproteinizing rna is by rapid homogenization in the presence of guanidinium thiocyanate or guanidine hydrochloride macdonald et al. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Guanidine hydrochloride material safety data sheet cas no 50. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Isolation of intact rna, free of protein and dna, is the critical first step in analyzing steadystate levels of transgene mrna. Add small amounts of water until solid is entirely dissolved 4. Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins.
Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the. Bp178500 casno 50011 synonyms no information available recommended use laboratory chemicals. Identification of the substancemixture and of the companyundertaking 1. It can be used as a medicine, organic synthesis intermediate and is used in dyes. Use of guanidine hydrochloride guanidine hcl, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant. It is not indicated for treating myasthenia gravis. No wasting valuable research time weighing and dissolving guanidine crystals free from uv absorbing materials in the range of 225300nm free from heavy metal contaminants fr. Guanidine thiocyanate sc202638 hazard alert code key. If the patient feels unwell or is concerned, obtain medical advice.
Safe use of guanidine hydrochloride in pregnancy has not been established. It may appear as a freeflowing crystalline powder, a freeflowing powder with solid material dispersed throughout, or a solid. Material may be irritating to the mucous membranes and upper respiratory tract. Clp ghs the criteria for classification are not fulfilled. Measure concentration of solution by refractive index measurements pace cn, meth. Guanidine hcl is also applied as a solubilizing agent in electrophoresis and in molecular weight determination. Carbon oxides, nitrogen oxides nox, hydrogen chloride gas. Other information revision 061917 modified effective date the information contained in this data sheet is based on the data available to puritan products at this time. Safety data sheet guanidine hydrochloride biospectra. Thermo scientific pierce 8m guanidine hcl solution is an accurately formulated, readytouse solution of guanidine hydrochloride, that can be easily diluted and phadjusted to any concentration below 8m.